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Cloning of an isoform of mouse TGF‐β type II receptor gene
Author(s) -
Suzuki Atsushi,
Shioda Noriko,
Maeda Toshihiko,
Tada Masazumi,
Ueno Naoto
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01156-7
Subject(s) - gene isoform , alternative splicing , microbiology and biotechnology , biology , complementary dna , acvr2b , receptor , transforming growth factor , gene , tgf beta signaling pathway , genetics
A variant of transforming growth factor‐β type II receptor (TGF‐βRII) cDNA was isolated from a mouse brain cDNA library. The predicted receptor is identical to previously reported mouse TGF‐βRII except that the isoform has an insertion sequence of 25 amino acids in the predicted ligand‐binding domain. By the use of reverse transcription‐polymerase chain reaction (RT‐PCR), transcripts for both isoforms were detected in all tissues and developing embryos examined. The isoform transiently expressed in COS cells showed a similar ligand‐binding specificity to authentic TGF‐βRII. These results suggest that the mouse TGF‐βRII gene generates multiple isoforms, possibly by alternative splicing, as reported for activin type IIB receptor; and an isoform which has the extra sequence in the ligand‐binding domain is also involved in the TGF‐β signal transduction.