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A 1 H NMR NOE study on Co(II) stellacyanin: Some clues about the structure of the metal site
Author(s) -
Vila Alejandro J.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01141-9
Subject(s) - chemistry , metal , ligand (biochemistry) , proton , solvent , crystallography , proton nmr , hyperfine structure , nuclear magnetic resonance spectroscopy , stereochemistry , organic chemistry , physics , atomic physics , biochemistry , receptor , quantum mechanics
The 1 H NMR spectrum of Co(II) stellacyanin is reported, in which four signals not previously observed have been detected. NOE experiments were performed to assign the hyperfine shifted signals corresponding to a Cys and two His residues. Both His residues are solvent‐accessible and are shown to bind the metal ion through their Nδ1 atoms. The β‐CH 2 Cys proton shifts indicate the presence of a strong axial ligand.
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