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pH‐independent inhibition of restriction endonuclease cleavage via triple helix formation by oligonucleotides containing 8‐oxo‐2′‐deoxyadenosine
Author(s) -
Wang Qing,
Tsukahara Satoru,
Yamakawa Hidefumi,
Takai Kazuyuki,
Takaku Hiroshi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01139-7
Subject(s) - deoxyadenosine , triple helix , oligonucleotide , cleavage (geology) , chemistry , restriction enzyme , endonuclease , stereochemistry , dna , enzyme , recognition sequence , biochemistry , nucleotide , biology , gene , paleontology , fracture (geology)
The ability of homopyrimidine oligonucleotides containing 8‐oxo‐2′‐deoxyadenosine to form stable, triple helical structures with the sequence containing the recognition site for the class II‐S restriction enzyme, Ksp 632‐I, was studied as a function of pH. The 8‐oxo‐2′‐deoxyadenosine‐substituted oligomers were shown to inhibit enzymatic cleavage and to bind within the physiological pH range in a pH‐independent fashion without compromising specificity.