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Enhancing effect of the 3′‐untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro
Author(s) -
Zeyenko Vladimir V.,
Ryabova Lyubov A.,
Gallie Daniel R.,
Spirin Alexander S.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01126-5
Subject(s) - tobacco mosaic virus , in vitro , rna , virology , chemistry , virus , biology , microbiology and biotechnology , biochemistry , gene
In order to test the enhancing effect of the 3′‐terminal untranslated region (3′‐UTR) of tobacco mosaic virus (TMV) RNA on protein synthesis in vitro we used a chimeric mRNA construct containing TMV 5′‐UTR (Ω) and firefly luciferase mRNA. The addition of the TMV 3′‐UTR to the chimeric mRNA construct results in a more than 3‐fold stimulation of the synthesis of the functionally active protein in the wheat germ cell‐free translation system. We have demonstrated that the proper length of the TMV 3′‐terminal part is important for efficient translation; elongation of the TMV tail by 160 vector‐derived nucleotides fully abolishes the stimulation effect of the TMV 3′‐UTR in vitro.