Premium
Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity
Author(s) -
Machray Gordon C.,
Burch L.,
Hedley P.E.,
Davies H.V,
Waugh R.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01105-2
Subject(s) - complementary dna , solanum tuberosum , cloning (programming) , biology , gene , cdna library , fusion protein , microbiology and biotechnology , ferredoxin , dna , rapid amplification of cdna ends , molecular cloning , enzyme , biochemistry , botany , recombinant dna , computer science , programming language
The cloning of a 1332 bp cDNA from a potato ( Solanum tuberosum L.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum . Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.