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RNA/DNA hybrid duplexes with identical nearest‐neighbor base‐pairs have identical stability
Author(s) -
Sugimoto Naoki,
Katoh Misa,
Nakano Shu-ichi,
Ohmichi Tatsuo,
Sasaki Muneo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01098-6
Subject(s) - k nearest neighbors algorithm , base pair , rna , dna , melting temperature , nucleic acid secondary structure , chemistry , physics , gene , materials science , biochemistry , computer science , artificial intelligence , composite material
Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest‐neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest‐neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes.