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Identification of two ets related genes in a marine worm, the polychaete annelid Nereis diversicolor
Author(s) -
Lelièvre-Chotteau Anne,
Laudet Vincent,
Flourens Anne,
Bègue Agnès,
Leprince Dominique,
Fontaine François
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01091-9
Subject(s) - annelid , polychaete , biology , gene , ets transcription factor family , genetics , lineage (genetic) , dna binding domain , transcription factor , evolutionary biology , microbiology and biotechnology , zoology , ecology
The ETS family includes a growing number of transcription factors with a highly conserved DNA‐binding domain, the ETS domain. We have used PCR amplification with degenerated oligonucleotides to isolate two putative ETS DNA‐binding coding domains in a primitive form of cœlomate, the polychaete annelid Nereis diversicolor . These sequences are highly related to the ETS and ERG groups of the ets gene family. For the erg sequence an adjacent region encoding for 91 amino acids has been characterized after library screening, and we show an expression in cells isolated from the cœlomic cavity of the animal. A phylogenic analysis confirms that the ets‐1/ets‐2 and the erg/fli dichotomy arose specifically in the vertebrate lineage.