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Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species
Author(s) -
Tullo Apollonia,
Tanzariello Filomena,
D'Erchia Anna Maria,
Nardelli Maria,
Papeo Pasquale Antonio,
Sbisà Elisabetta,
Saccone Cecilia
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01080-3
Subject(s) - polyadenylation , transcription (linguistics) , nadh dehydrogenase , transfer rna , messenger rna , rna , biology , gene , mitochondrial dna , gene expression , microbiology and biotechnology , antisense rna , genetics , linguistics , philosophy
We have characterized the transcriptional pattern of the rat mitochondrial ND6‐containing region in vivo. We have identified a stable polyadenylated RNA species complementary for the full length of the ND6 mRNA. The analysis of the ND5 region has revealed the presence of an antisense RNA only at its 3′ end. The presence of these stable antisense species complementary to structural genes is intriguing and suggests a possible regulatory function. The quantitative analyses have demonstrated that the H transcripts, both codogenic and non‐codogenic, are more stable than the L transcripts. We have defined the 5′ end of the ND6 mRNA at the level of the ATG downstream of the tRNA Glu . The mapping of the ND1 5′ end has demonstrated that GTG is the first codon of the mRNA. Our findings suggest that the post‐transcriptional mechanisms involved in the expression of the mt genome are much more numerous and complex than those already described in the literature.