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cDNA cloning of a cytosolic protein tyrosine phosphatase (RKPTP) from rat kidney
Author(s) -
Moriyama Toshiki,
Kawanishi Sachio,
Inoue Takuya,
Imai Enyu,
Kaneko Tetsuya,
Xia Chen,
Takenaka Masaru,
Noguchi Tamio,
Kamada Takenobu,
Ueda Naohiko
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01064-1
Subject(s) - complementary dna , protein tyrosine phosphatase , biology , microbiology and biotechnology , biochemistry , peptide sequence , open reading frame , tyrosine , gene
A rat cDNA encoding a non‐receptor type phosphotyrosine phosphatase (PTPase; EC 3.1.3.48) was identified. The 1608 bp cDNA contains a single open reading frame that predicts a 382 amino acid protein with M r 44,438. The predicted protein has no apparent signal or transmembrane sequences, suggesting that it is a cytosolic protein. The C‐terminal region has a PTPase catalytic domain that has 40–50% nucleic acid homology to other known PTPases. The N‐terminal region has little amino acid sequence homology to any other known sequences. The recombinant protein of the cloned cDNA expressed in Escherichia coli was shown to possess PTPase activity using myelin basic protein, tyrosine phosphorylated by p43 ν‐ abl tyrosine kinase, as a substrate.