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Expression of GATA‐binding transcription factors in rat hepatocytes
Author(s) -
Matsuda Koichi,
Kobune Yoshiko,
Noda Chiseko,
Ichihara Akira
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01062-5
Subject(s) - microbiology and biotechnology , gata2 , gene , biology , complementary dna , gata transcription factor , serine , dna binding protein , gene expression , transcription (linguistics) , fusion protein , transcription factor , biochemistry , promoter , recombinant dna , phosphorylation , linguistics , philosophy
Recently, we demonstrated that a DNA‐binding protein(s) is involved in transcriptional repression of the rat serine dehydratase gene in fetal liver. Here, we report that a GAT(A/T) motif is the target sequence for the DNA‐binding protein. By screening a fetal liver cDNA library, we isolated a rat homolog of GATA‐1. Rat GATA‐1 expressed as a GST‐fusion protein in E. coli bound to the GAT(A/T) motif in the serine dehydratase gene. Northern analysis show that GATA‐1 and GATA‐4 mRNAs are expressed in fetal hepatocytes.