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Visualisation by electron microscopy of the unique part of the cytoplasmic domain of a desmoglein, a cadherin‐like protein of the desmosome type of cell junction
Author(s) -
Rutman A.J.,
Buxton R.S.,
Burdett I.D.J.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01049-8
Subject(s) - desmosome , desmoglein , cadherin , cytoplasm , electron microscope , chemistry , peptide sequence , biophysics , microbiology and biotechnology , biology , physics , cell , biochemistry , optics , gene
Part of the cytoplasmic domain of a human desmoglein, Dsgl, a cadherin‐like protein found in desmosomes of epithelial cells, has been visualised by electron microscopy. The cloned fragment contains five repeats of a 29 ± 4 residue sequence unique to desmogleins, followed by a glycine‐rich region. In rotary shadowed preparations the molecule consists of a globular head attached to a thin tail, the latter perhaps corresponding to the glycine‐rich region. This portion of the molecule is thought to span the width of the inner dense plaque. The structure and dimensions concur well to the configuration deduced from the protein sequence.