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Yta10p, a member of a novel ATPase family in yeast, is essential for mitochondrial function
Author(s) -
Tauer Reimund,
Mannhaupt Gertrud,
Schnall Ralf,
Pajic Alexander,
Langer Thomas,
Feldmann Horst
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01045-5
Subject(s) - yeast , biology , biochemistry , aaa proteins , mitochondrion , atpase , saccharomyces cerevisiae , respiratory chain , peptide sequence , amino acid , gene , atp–adp translocase , proteases , mitochondrial carrier , inner mitochondrial membrane , bacterial outer membrane , enzyme , escherichia coli
The yeast gene, YTA10 , encodes a member of a novel family of putative ATPases. Yta10p, as deduced from the nucleotide sequence, is 761 amino acids in length (predicted molecular mass 84.5 kDa). The amino acid sequence of Yta10p exhibits high similarity to two other yeast proteins, Yta11 and Yta12, and to E coli FtsH. Several features of Yta10p are compatible with its localization in mitochondria. We report here that Yta10p is a yeast mitochondrial protein and that import is dependent on a membrane potential and accompanied by processing to a protein of approximately 73 kDa. Disruption of YTA10 leads to a nuclear petite phenotype and to a loss of respiratory competence, as shown by spectrophotometric measurement of the activities of respiratory complexes I–III and IV, respectively. These findings together with the high similarity of Yta10p to several ATP‐dependent proteases suggest that Yta10p is a mitochondrial component involved, directly or indirectly, in the correct assembly and/or maintenance of active respiratory complexes.