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Equivalent regulation of wild type and an epitope‐tagged variant of G s α by the IP prostanoid receptor following expression in neuroblastoma × glioma hybrid, NG108‐15, cells
Author(s) -
Mullaney Ian,
Milligan Graeme
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01043-9
Subject(s) - prostanoid , epitope , glioma , receptor , neuroblastoma , chemistry , cancer research , microbiology and biotechnology , biology , genetics , biochemistry , antibody , cell culture
NG108‐15 cells were transfected to stably express a haemagglutinin epitope‐tagged variant of the long isoform of G s α. Clone BST15 expressed this polypeptide at similar levels to the endogenous long isoform of G s α. Treatment of clone BST15 with the IP prostanoid receptor agonist iloprost resulted in down‐regulation of both forms of G s α with both dose—effect curves to iloprost and time courses of loss of the two forms of G s α being indistinguishable. These results demonstrate that the IP prostanoid receptor interacts with and regulates the epitope‐tagged variant of G s α in an equivalent manner to the unmodified protein and indicates that the epitope‐tagged polypeptide can be used to analyse mechanisms of receptor regulation of cellular G‐protein levels.