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Structure of the gene for human uracil—DNA glycosylase and analysis of the promoter function
Author(s) -
Haug Terje,
Skorpen Frank,
Lund Henning,
Krokan Hans E.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01042-0
Subject(s) - dna glycosylase , tata box , gene , promoter , caat box , uracil dna glycosylase , microbiology and biotechnology , biology , transcription (linguistics) , exon , dna , genetics , gene expression , dna repair , linguistics , philosophy
The gene for human uracil‐DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SPI elements and a putative c‐MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full‐length construct. A region upstream of these elements appears to exert a negative control function.