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Calcium‐sensitive control of rhodopsin phosphorylation in the reconstituted system consisting of photoreceptor membranes, rhodopsin kinase and recoverin
Author(s) -
Gorodovikova Ele.,
Senin Ivan I.,
Philippov Pavel P.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01030-7
Subject(s) - recoverin , rhodopsin , kinase , phosphorylation , biochemistry , chemistry , biophysics , myristoylation , microbiology and biotechnology , biology , retinal
Rhodopsin phosphorylation in the reconstituted system consisting of urea‐washed photoreceptor membranes, rhodopsin kinase and recoverin is regulated by Ca 2+ : the process takes place at low [Ca 2+ ] but is suppressed at high [Ca 2+ ]. In the absence of recoverin, rhodopsin kinase is active irrespective of the cation concentration used. Hence, recoverin is an inhibitor (at high [Ca 2+ ]) but not an activator of rhodopsin kinase. Based jointly on these data obtained on the reconstituted system and on our preceding experiments on rod outer segments suspension, one may conclude that (i) the function of recoverin in retina rod cells is the Ca 2+ ‐sensitive control of rhodopsin phosphorylation and (ii) the presence of recoverin is essential and sufficient to provide rhodopsin kinase with the Ca 2+ sensitivity.