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Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen
Author(s) -
Namba Motoshi,
Kurose Mayumi,
Torigoe Kakuji,
Hino Katsuhiko,
Taniguchi Yoshifumi,
Fukuda Shigeharu,
Usui Mitsuko,
Kurimoto Masashi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01022-6
Subject(s) - cryptomeria , complementary dna , allergen , open reading frame , peptide sequence , homology (biology) , biology , pollen , microbiology and biotechnology , amino acid , japonica , biochemistry , botany , gene , allergy , immunology
Cloning of a cDNA from Cry j II, the second major allergen from Japanese cedar ( Cryptomeria japonica ) pollen, is described. An isolated Cry j II cDNA contained an open reading frame coding for 514 amino acid residues. The mature Cry j II protein consisted of 388 amino acid residues (R 46 —S 433 ). According to a homology analysis, no amino acid sequence homology was observed between Cry j II and Cry j I, another major allergen. But Cry j II showed homology with polygalacturonase (PG) derived from tomato (40% identity) at the amino acid level. The sequence information can potentially be used to devise an effective course of immunotherapy for Japanese cedar pollinosis.