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Molecular cloning and chromosomal localization of the human thrombopoietin gene
Author(s) -
Sohma Yoshiaki,
Akahori Hiromichi,
Seki Naohiko,
Hori Tada-aki,
Ogami Kinya,
Kato Takashi,
Shimada Yoshihiro,
Kawamura Kazuo,
Miyazaki Hiroshi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01008-0
Subject(s) - thrombopoietin , molecular cloning , gene , cloning (programming) , biology , genetics , microbiology and biotechnology , complementary dna , stem cell , haematopoiesis , computer science , programming language
The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS‐1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin‐labeled probe.