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Ganglioside antigen of DU‐PAN‐2 in a human pancreatic cancer
Author(s) -
Hamanaka Yuichiro,
Hamanaka Sumiko,
Shinagawa Yuji,
Suzuki Takashi,
Inagaki Fuyuhiko,
Suzuki Minoru,
Suzuki Akemi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01004-8
Subject(s) - immunostaining , ganglioside , chemistry , microbiology and biotechnology , antigen , pancreatic cancer , enzyme , adenocarcinoma , biochemistry , cancer , biology , immunohistochemistry , immunology , genetics
DU‐PAN‐2 reactive gangliosides were isolated from the tumor of a patient with pancreatic cancer (duct cell carcinoma, moderately differentiated adenocarcinoma), having a negative Lewis blood phenotype, and were analyzed by means of TLC‐immunostaining, enzyme‐linked immunosorbent assay (ELISA), permethylation study, 1 H NMR spectroscopy and fast atom bombardment mass spectrometry. The structures of the gangliosides were found to be NeuAcα2‐3Ga1β1‐3G1cNAcβ1‐3Ga1β1‐4Glcβ1‐1′Cer, containing normal and hydroxy fatty acids. By TLC‐immunostaining and ELISA with chemically synthesized gangliosides, DU‐PAN‐2 was demonstrated to react strongly with IV 3 αNeuAc‐Lc 4 Cer, weakly with IV 3 αNeuAc‐nLc 4 Cer, and moderately with IV 6 αNeuAc‐Lc 4 Cer and IV 6 αNeuAc‐nLc 4 Cer. Thus it was concluded that the DU‐PAN‐2 reactive ganglioside in the tumor is IV 3 αNeuAc‐Lc 4 Cer and that DU‐PAN‐2 has a rather broad specificity.