Premium
Electrophoretic separation of developmental and adult rabbit skeletal muscle myosin heavy chain isoforms: Example of application to muscle denervation study
Author(s) -
Janmot Chantal,
d' Albis Anne
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01002-1
Subject(s) - myosin , denervation , skeletal muscle , gene isoform , major histocompatibility complex , biology , electrophoresis , microbiology and biotechnology , regeneration (biology) , heavy chain , chemistry , anatomy , biochemistry , antigen , immunology , gene
We present the separation by SDS gel‐electrophoresis of the six main myosin heavy chains (MHC) present in rabbit skeletal muscle. The separation of the four adult MHC (1, 2A, 2X/2D, 2B) was compared to that of the corresponding rat MHC as described by Talmadge and Roy [J. Appl. Physiol. 99 (1993) 2337‐2340]. We found that many rabbit muscles contained mainly one of the four MHC, in some cases the 2B MHC. In addition, we resolved the embryonic E and perinatal P developmental MHC, which should facilitate muscle differentiation and regeneration studies in the rabbit. An example of application to the study of muscle denervation is given.