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Effect of unmodified triple helix‐forming oligodeoxyribonucleotide targeted to human multidrug‐resistance gene mdr1 in MDR cancer cells
Author(s) -
Scaggiante Bruna,
Morassutti Carla,
Tolazzi Giuseppe,
Michelutti Angela,
Baccarani Michele,
Quadrifoglio Franco
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00995-3
Subject(s) - triple helix , gene , coding region , multiple drug resistance , microbiology and biotechnology , oligonucleotide , biology , messenger rna , gene expression , cell culture , transmembrane domain , drug resistance , cancer research , chemistry , genetics
The human mdr1 gene encodes a transmembrane glycoprotein the over‐expression of which is associated with development of multidrug resistance in human tumor cells. A negative modulation of human mdr1 has been attempted via a 27‐mer unmodified triple helix‐forming oligonucleotide, named 1D, targeted to a homopurine sequence in the coding region of the gene. By administering 10 μM of 1D we could find a significant reduction in MDR1 mRNA levels in the human drug‐resistant cell line CEM‐VLB 100. This effect appears to be specific and due to a transient block of RNA polymerase mediated by triple helix formation.