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Determination of the vitronectin binding site on plasminogen activator inhibitor 1 (PAI‐1)
Author(s) -
van Meijer Marja,
Gebbink Raymond Klein,
Preissner Klaus T.,
Pannekoek Hans
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00990-2
Subject(s) - vitronectin , plasminogen activator inhibitor 1 , chemistry , binding site , plasminogen activator , biochemistry , microbiology and biotechnology , medicine , biology , integrin , receptor
Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI‐1). We used a well‐characterized panel of anti‐human PAI‐1 monoclonal antibodies (MoAbs) to localize the vitronectin‐binding site on PAI‐1. By employing a direct vitronectin/PAI‐1 binding assay and two vitronectin‐dependent inhibition assays, we demonstrate that the anti‐PAI‐1 MoAbs CLB‐5, CLB‐10, CLB‐2C8 and I1, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI‐1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI‐1 harbours an important determinant for the interaction with vitronectin.