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The immediate vicinity of mouse metallothionein‐I gene contains two sites conferring glucocorticoid inducibility to the heterologous promoter
Author(s) -
Plisov Sergey Y.,
Nichiporenko Marina G.,
Shkapenko Alla L.,
Kumarev Victor P.,
Baranova Ludmila V.,
Merkulova Tatyana I.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00980-5
Subject(s) - glucocorticoid , heterologous , promoter , gene , microbiology and biotechnology , transfection , transcription (linguistics) , metallothionein , biology , transcription factor , chemistry , gene expression , genetics , immunology , linguistics , philosophy
Glucocorticoid responsive elements (GREs) located −252 to −209 bp upstream and +1011 to +1054 bp downstream of the transcription initiation site of the mouse metallotionein‐I (mMT‐I) gene were identified in transient transfection eperiments. However, the promoter region of the mMT‐I gene (−330 to +70 bp) was found to provide low, if any, glucocorticoid induction of the linked CAT gene, while showing strong cadmium regulation, comparable with the in vivo level.