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Functional interaction between the epidermal growth factor receptor and c‐Src kinase activity
Author(s) -
Oude Weernink Paschal A.,
Ottenhoff-Kalff Astrid E.,
Vendrig Marijke P.,
van Beurden Ellen A.C.M.,
Staal Gerard E.J.,
Rijksen Gert
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00977-5
Subject(s) - epidermal growth factor , tyrosine kinase , proto oncogene tyrosine protein kinase src , transfection , microbiology and biotechnology , cell culture , biology , epidermal growth factor receptor , kinase , chemistry , receptor , signal transduction , biochemistry , genetics
To study the relationship between the tyrosine kinase c‐Src and the epidermal growth factor receptor (EGF‐R), we used the breast cancer cell line ZR75‐1, which was transfected with the EGF‐R. The EGF‐R transfected cell line expressed 60 times more EGF‐R than a control cell line transfected with the empty vector. In the presence of EGF, the EGF‐R over‐expressing cell line grew much faster than the control cell line. Both cell lines expressed approximately equal amounts of c‐Src. However, the cell line over‐expressing the EGF‐R showed a twofold enhancement of c‐Src kinase activity after EGF stimulation. The activation of c‐Src kinase by EGF was confirmed in other EGF‐R expressing cell types.