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A herbicide antidote (safener) induces the activity of both the herbicide detoxifying enzyme and of a vacuolar transporter for the detoxified herbicide
Author(s) -
Gaillard Cécile,
Dufaud Annie,
Tommasini Roberto,
Kreuz Klaus,
Amrhein Nikolaus,
Martinoia Enrico
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00961-9
Subject(s) - vacuole , glutathione , chemistry , transporter , conjugate , enzyme , biochemistry , stimulation , biology , cytoplasm , gene , mathematical analysis , mathematics , neuroscience
In plants potentially toxic compounds are ultimately deposited in the large central vacuole. In this report we show that isolated barley mesophyll vacuoles take up the glucoside conjugate of the herbicide derivate [5‐hydroxyphenyl]primisulfuron. Transport is stimulated by Mg‐ATP and is distinct from that previously described for glutathione conjugates. Treatment of barley with different herbicide antidotes (safeners) revealed that the safener cloquintocet‐mexyl doubles the vacuolar transport activities for both the glutathione and glucoside conjugates. Stimulation of the uptake of the metolachlor—glutathione conjugate was the result of an increased uptake velocity whereas the K m remained unaltered, suggesting that the higher activity was due to a higher expression of the transporter. These results indicate that modulation of vacuolar transport activities are an integral part of the detoxification mechanism of plants.