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Cloning of a Xenopus laevis muscarinic receptor encoded by an intronless gene
Author(s) -
Herrera Luisa,
Carvallo Pilar,
Antonelli Marcelo,
Olate Juan
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00957-0
Subject(s) - xenopus , complementary dna , biology , microbiology and biotechnology , muscarinic acetylcholine receptor , gene , cdna library , amino acid , molecular cloning , receptor , genetics
The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identity of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a muscarinic receptor (xMR). The deduced amino acid sequence reveals a protein of 484 residues with an apparent molecular weight of 54,188 Da. Amino acid comparison with previously cloned mammalian muscarinic receptors showed a 78% identity with the human m4 subtype, presenting at the same time clustered differences within the amino‐terminal region and third intracellular loop. Genomic Southern analysis displayed the presence of one main gene belonging to this subtype, and the PCR analysis revealed an intronless gene.