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T cell epitope analysis with peptides simultaneously synthesized on cellulose membranes: Fine mapping of two DQ dependent epitopes
Author(s) -
Adler Sabine,
Frank Ronald,
Lanzavecchia Antonio,
Weiss Siegfried
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00950-3
Subject(s) - epitope , peptide , chemistry , epitope mapping , membrane , amino acid , major histocompatibility complex , microbiology and biotechnology , biochemistry , antigen , biology , gene , immunology
Several MHC class II restricted, CD4 + human T cell clones from three donors were induced with soluble matrix protein of influenza virus. The epitopes recognized by these clones were mapped using a complete set of overlapping 15‐mer peptides synthesized with the newly developed SPOT method which allows the simple simultaneous synthesis of numerous peptides on cellulose membranes. Fine analysis of two clones by truncation of the stimulatory peptide by single subsequent amino acids from either the NH 2 ‐ or the COOH‐terminus revealed the minimal stimulatory determinants of two DQ dependent T cell epitopes.