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The regulation of sodium‐dependent transport of anionic amino acids in cultured human fibroblasts
Author(s) -
Franchi-Gazzola Renata,
Visigalli Rossana,
Bussolati Ovidio,
Gazzola Gian C.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00929-5
Subject(s) - protein kinase c , bradykinin , stimulation , chemistry , amino acid , sodium , biochemistry , platelet derived growth factor receptor , microbiology and biotechnology , phosphorylation , endocrinology , biology , receptor , growth factor , organic chemistry
In cultured human fibroblasts the transport of anionic amino acids through the sodium‐dependent system X − AG is stimulated rapidly and transiently by phorbol 12,13‐dibutyrate. Transport stimulation is consistent with an effect due to the activation of protein kinase C. Bradykinin (1 μM) and PDGF‐AA (100 ng/ml) also stimulate the activity of system X − AG . The bradykinin effect appears to be fully dependent upon PKC activation whereas the stimulation of aspartate transport by PDGF‐AA is also due to PKC‐independent mechanisms.