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Distribution and characterization of immunoreactive rat adrenomedullin in tissue and plasma
Author(s) -
Sakata Junichiro,
Shimokubo Toru,
Kitamura Kazuo,
Nishizono Masatoshi,
Iehiki Yoshinari,
Kangawa Kenji,
Matsuo Hisayuki,
Eto Tanenao
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00928-7
Subject(s) - adrenomedullin , chemistry , distribution (mathematics) , tissue distribution , medicine , biophysics , biochemistry , biology , peptide , mathematical analysis , mathematics
Adrenomedullin is a new bioactive peptide recently isolated from pheochromocytoma. We report on the rat adrenomedullin distribution and molecular forms in various tissues and plasma. Using a sensitive radioimmunoassay system for rat adrenomedullin, high concentrations of immunoreactive rat adrenomedullin were detected in adrenal gland, lung and cardiac atrium. In lung and atrium, the immunoreactivity concentration in rat was about 6–10 times higher than that in human. The mean plasma concentration of immunoreactive rat adrenomedullin was 3.60 ±0.34 fmol/ml (mean ± S.D.). Analysis in adrenal gland, lung and atrium with reverse‐phase and gel‐filtration high‐performance liquid chromatography showed that most immunoreactive rat adrenomedullin emerged as a single peak at a position exactly identical to that of the authentic rat adrenomedullin peptide, synthesized according to the sequence predicted from the cDNA.