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Absorption flattening in the optical spectra of liposome‐entrapped substances
Author(s) -
Wittung Pernilla,
Kajanus Johan,
Kubista Mikael,
Malmström Bo G.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00912-0
Subject(s) - chromophore , liposome , molar absorptivity , flattening , chemistry , absorption (acoustics) , vesicle , molecule , absorption spectroscopy , photochemistry , phospholipid , analytical chemistry (journal) , chemical physics , materials science , chromatography , optics , organic chemistry , physics , membrane , biochemistry , composite material
Measuring optical spectra of chromophore molecules entrapped in liposomes can lead to considerable distortion because of absorption flattening. This phenomenon is analyzed theoretically, and it is shown that the deviation from the Beer—Lambert law becomes larger as the molar absorptivity of the chromophore increases and as the number of entrapped molecules becomes larger. The theoretical equations are tested experimentally with intermediate‐sized phospholipid vesicles containing entrapped cytochrome c . It is shown that considerable absorption flattening is observed with vesicles containing about 50 chromophore molecules. The equation given can be used to correct the spectrum.