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Activation of the alternative pathway of complement by apoptotic Jurkat cells
Author(s) -
Matsui Hiroyuki,
Tsuji Shoutaro,
Nishimura Hitosi,
Nagasawa Shigeharu
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00897-3
Subject(s) - jurkat cells , ic3b , alternative complement pathway , microbiology and biotechnology , opsonin , apoptosis , complement receptor , complement system , classical complement pathway , biology , factor h , chemistry , antibody , t cell , immunology , phagocytosis , immune system , biochemistry
Jurkat T cells die of apoptosis upon exposure to anti‐Fas mAb. Here we show that although the alternative complement pathway generally does not attack homologous cells, anti‐Fas‐induced apoptotic Jurkat T cells were attacked antibody‐independently by the alternative pathway of human complement and opsonized with iC3b, which is a ligand of the complement receptor type 3 (CR3) of phagocytes. These results suggest that apoptotic cells become the targets of the homologous alternative complement pathway, which facilitates the clearance of apoptotic cells by phagocytes.