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Molecular cloning and evolutional analysis of a mammalian homologue of the Distal‐less 3 ( Dlx‐3 ) homeobox gene
Author(s) -
Shirasawa Takuji,
Sakamoto Kyoichi,
Tkahashi Hiroshi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00896-5
Subject(s) - homeobox , emx2 , homeobox a1 , homeobox protein nkx 2.5 , biology , hnf1b , complementary dna , genetics , gene , open reading frame , cdna library , cloning (programming) , nucleic acid sequence , sequence analysis , peptide sequence , molecular cloning , microbiology and biotechnology , gene expression , computer science , programming language
We have cloned and sequenced a cDNA encoding the mammalian homologue of Distal‐less 3 ( Dlx‐3 ) from a rat embryonic brain cDNA library. The primary structure of rat Dlx‐3 showed a cDNA with an open reading frame of 290 amino acids with a molecular weight of 31 kDa harboring a homeodomain sequence characteristic of the Distal‐less homoeobox gene. In addition to the highly conserved homeodomain sequence, we newly found the consensus motifs in the N‐terminal and C‐terminal region, which were specifically conserved among Distal‐less members of different species. Phylogenetic analysis of the Distal‐less homeodomain sequence also showed a possible association between the sequence similarities in the homeodomain and the spatial specifications of homeobox genes expressed in the developing central nervous system.