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An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase
Author(s) -
Saiki Keitarou,
Mogi Tatsushi,
Ishizuka Morio,
Anraku Yasuhiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00889-2
Subject(s) - escherichia coli , thermophile , microbiology and biotechnology , gene , atp synthase , biology , heme , escherichia , genetics , chemistry , bacteria , biochemistry , enzyme
The cyoE gene of the Escherichia coli bo ‐type quinol oxidase operon ( cyo ABCDE ) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa 3 ‐type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic complementation analysis using the chimeric operon cyo ABCD‐caaE and heme O synthase assay using the CaaE‐overproduced E. coli membranes. We found that the caaE gene encodes a thermotolerant heme O synthase which provides an intermediate for heme A biosynthesis.