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Molecular cloning of a novel candidate G protein‐coupled receptor from rat brain
Author(s) -
Song Z.H.,
Young W.Scott,
Brownstein Michael J.,
Bonner Tom I.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00888-4
Subject(s) - receptor , biology , g protein coupled receptor , complementary dna , amino acid , microbiology and biotechnology , transmembrane domain , cloning (programming) , molecular cloning , subfamily , rhodopsin like receptors , transmembrane protein , biochemistry , gene , metabotropic receptor , glutamate receptor , programming language , computer science
A PCR cloning strategy using primers designed from sequences selectively conserved among a cannabinoid receptor and two orphan receptors, was used to isolate novel G protein‐coupled receptors. rCNL3, a 1.75 kb cDNA encoding a 363 amino acid protein, was isolated from a rat cerebral cortex library. Sequence analysis showed that rCNL3 possesses a number of structural characteristics of G protein‐coupled receptors and has 61% amino acid identity (from transmembrane region one through the carboxyl‐terminus) with two other candidate G protein‐coupled receptors. Therefore, these three receptors may comprise a receptor subfamily with identical or closely related endogenous ligands. Northern and in situ hybridization experiments demonstrated that rCNL3 mRNA is expressed in the rat brain, with a prominent distribution in striatum.