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Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O ‐acetylserine (thiol) lyase from Arabidopsis thaliana
Author(s) -
Hell Rüdiger,
Bork Christiane,
Bogdanova Natascha,
Frolov Igor,
Hauschild Rüdiger
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00872-8
Subject(s) - gene isoform , arabidopsis thaliana , biochemistry , lyase , cytosol , mutant , cysteine , plastid , arabidopsis , biology , microbiology and biotechnology , gene , chemistry , chloroplast , enzyme
cDNAs encoding for two isoforms of O ‐acetylserine (thiol) lyase (OAS‐TL), which catalyzes the synthesis of cysteine, have been isolated from Arabidopsis thaliana . Secondary structure together with expression patterns derived during photomorphogenesis indicate cellular localizations in the cytosol and plastids, thus allowing a direct comparison of compartment‐specific forms within one species. The cytosolic OAS‐TL complemented an E. coli auxotrophic mutant lacking cysteine synthesis. Both isoforms are represented by small gene families. They are expressed under all conditions investigated and were observed to increase in expression in plants grown with limited sulfate supply.