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Fluorescent differential display: Arbitrarily primed RT‐PCR fingerprinting on an automated DNA sequencer
Author(s) -
Ito Takashi,
Kito Keiji,
Adati Naoki,
Mitsui Yuko,
Hagiwara Hisashi,
Sakaki Yoshiyuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00867-1
Subject(s) - dna sequencer , fluorescence , dna , dna profiling , chemistry , microbiology and biotechnology , biology , dna sequencing , biochemistry , physics , optics
We established robust, reliable protocols for ‘Differential Display (DD),’ an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967–971] using RT‐PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such ‘Fluorescent Differential Display (FDD)’ techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.