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Vitamin D influences gap junctional communication in C3H/10T 1/2 murine fibroblast cells
Author(s) -
Stahl Wilhelm,
Nicolai Swantje,
Hanusch Michael,
Sies Helmut
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00858-2
Subject(s) - retinoic acid , gap junction , lucifer yellow , vitamin , retinoid , vitamin d and neurology , tretinoin , intracellular , endocrinology , biology , medicine , fibroblast , microbiology and biotechnology , cell culture , cholecalciferol , cell–cell interaction , chemistry , cell , biochemistry , genetics
Vitamin D3, cholecalciferol, induces cell—cell communication via gap junctions in murine fibroblasts (C3H/10T 1/2 cells) at concentrations between 0.01 and 1.0 μM, as assayed by the dye transfer method using Lucifer yellow CH. The extent of induction is similar to that obtained with the positive controls, canthaxanthin or retinoic acid, applied at 10 and 1 μM, respectively. Vitamin D2 also induces cell—cell communication. At elevated concentrations of vitamin D3 (5 μM) there is a suppression of gap junctional communication, reversible upon exposure to all‐trans retinoic acid (1 μM) after removal of vitamin D3 from the medium. Conversely, communication between cells prestimulated with retinoic acid (1 μM) rapidly decreases when the retinoid is replaced by vitamin D3 (5 μM). The data demonstrate a role for vitamin D in the regulation of intercellular communication. This novel property of vitamin D may contribute to the antiproliferative effects of vitamin D exhibited in some types of cancer.