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Differentiation of HL‐60 cells is promoted by H‐toxin of Clostridium septicum
Author(s) -
Morinaga Naoko,
Kato Iwao,
Noda Masatoshi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00856-6
Subject(s) - adp ribosylation , toxin , clostridium septicum , dimethyl sulfoxide , chemistry , biochemistry , microbiology and biotechnology , biology , enzyme , nad+ kinase , organic chemistry
H‐toxin of Clostridium septicum potentiated dimethyl sulfoxide(DMSO)‐induced differentiation of human promyelocytic leukemia HL‐60 cells which were monitored by nuclear morphology and production of oxidative radicals. But, H‐toxin did not induce differentiation of HL‐60 cells in the absence of DMSO. These phenomena were not observed by staphylococcal leukocidin, a cytotoxin affecting to HL‐60 cells. In HL‐60 cells, ADP‐ribosylation of 118, 93, 75 and 58 kDa membrane proteins was observed, but the ADP‐ribosylation was not detected either in differentiated HL‐60 cells by DMSO or in normal polymorphonuclear leukocytes of human. When the membranes of HL‐60 cells were incubated with H‐toxin, ADP‐ribosylation of the membrane proteins was inhibited. Such suppressive effects on ADP‐ribosylation were not observed by DMSO or staphylococcal leukocidin. These data suggest that inhibition of the ADP‐ribosylation by H‐toxin may play an important role in potentiation of DMSO‐induced differentiation of HL‐60 cells.