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Oncogenically activated or ligand‐stimulated neu kinase stimulates neurite outgrowth in PC12 cells
Author(s) -
Gamett Daniel C.,
Cerione Richard A.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00855-8
Subject(s) - neurite , microbiology and biotechnology , chemistry , ligand (biochemistry) , kinase , receptor , biology , biochemistry , in vitro
Retroviral vectors pDOL/NeuN and pDOL/NeuT were used to express normal and transforming rat neu cDNAs in PC12 cells. DOL/ NeuT‐infected cells exhibited a high frequency of spontaneous neurite outgrowth while DOL/NeuN‐infected cells showed neurite outgrowth in the presence of heregulin, a putative ligand for the neu receptor tyrosine kinase. In both cases, neurite outgrowth was preceded by phosphorylation of p185 neu and several other cellular proteins. Thus the neu tyrosine kinase can elicit morphological and biochemical changes resembling, but distinct from, those stimulated by NGF, and heregulin stimulates neu to elicit these effects in PC12 cells.