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Oxygen‐derived radicals stimulate renin release of isolated juxtaglomerular cells
Author(s) -
Galle Jan,
Herzog Christel,
Schollmeyer Peter,
Wanner Christoph
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00853-1
Subject(s) - xanthine oxidase , renin–angiotensin system , catalase , superoxide dismutase , chemistry , reactive oxygen species , oxygen , juxtaglomerular apparatus , kidney , xanthine , biochemistry , incubation , endocrinology , medicine , enzyme , biology , organic chemistry , blood pressure
We assessed effects of reactive oxygen metabolites on renin release of juxtaglomerular cells (JG‐cells) prepared in primary culture from mouse kidneys. Renin activity was measured in culture supernatants and cells. Basal renin release was increased by incubation of JG‐cells with xanthine/xanthine oxidase from 26 ± 1% up to 58 ± 3% of total activity. This increase was slightly inhibited by superoxide dismutase, and was eliminated by addition of catalase, implicating H 2 O 2 as an intermediate product in the stimulatory cascade. H 2 O 2 applied exogeneously dose‐dependently stimulated renin release up to 55 ± 2%; this effect was also prevented by catalase. We propose that reactive oxygen metabolites stimulate renin release in isolated JG‐cells. This could have important implications in inflammatory kidney diseases.