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Interleukin 2‐induced activation of JAK3: Possible involvement in signal transduction for c‐ myc induction and cell proliferation
Author(s) -
Asao Hironobu,
Tanaka Nobuyuki,
Ishii Naoto,
Higuchi Masaya,
Takeshita Toshikazu,
Nakamura Masataka,
Shirasawa Takuji,
Sugamura Kazuo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00831-0
Subject(s) - signal transduction , microbiology and biotechnology , transfection , biology , mutant , receptor , gene , genetics
We have investigated the role of JAK3 in interleukin 2 (IL‐2)‐induced signal transduction with a human T cell line, ED40515(−), lacking expression of the IL‐2 receptor γ chain and its sublines transfected with wild‐type or mutant cDNAs of the IL‐2 receptor γ chain. Our results demonstrated that the membrane‐proximal cytoplasmic region, encompassing the src homology region 2 (SH2)‐like subdomain, of the γ chain is essential for association and activation of JAK3. Furthermore, IL‐2‐induced activation of JAK3 paralleled induction of the c‐ myc gene and DNA synthesis but not induction of the c‐ fos and c‐ jun genes. These results support the hypothesis that JAK3 plays a pivotal role in the IL‐2 receptor‐mediated signals for cell growth.