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Expression of a novel human myotonin protein kinase (MtPK) cDNA clone which encodes a protein with a thymopoietin‐like domain in COS cells
Author(s) -
Sasagawa Noboru,
Sorimachi Hiroyuki,
Maruyama Kei,
Arahata Kiichi,
Ishiura Shoichi,
Suzuki Koichi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00808-6
Subject(s) - complementary dna , clone (java method) , microbiology and biotechnology , protein kinase a , protein kinase domain , domain (mathematical analysis) , chemistry , kinase , biology , gene , biochemistry , mathematical analysis , mathematics , mutant
A full‐length cDNA of human myotonin protein kinase (MtPK) was cloned and expressed in COS‐1 cells. MtPK is recovered from the cytosolic fraction of the COS extract as a 70 kDa protein, which coincides with the size deduced from the predicted amino acid sequence. The sequence has a significant homology to thymopoietin, a peptide hormone of the thymus. Biochemical characteristics of MtPK expressed in COS‐1 cells and its expression in rat tissues are investigated.