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Nucleotide binding of the C‐terminal domains of the major histocompatibility complex‐encoded transporter expressed in Drosophila melanogaster cells
Author(s) -
Wang Kena,
Früh Klaus,
Peterson Per A.,
Yang Young
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00806-x
Subject(s) - drosophila melanogaster , terminal (telecommunication) , nucleotide , major histocompatibility complex , transporter , melanogaster , microbiology and biotechnology , genetics , biology , gene , telecommunications , computer science
The C‐terminal domains of the mouse transporter associated with antigen processing (TAP) were expressed as soluble proteins in Drosophila melanogaster cells and labeled by [α‐ 32 P]8‐azido‐ATP after UV‐irradiation. The relative potencies of the nucleotides in preventing azido‐ATP labeling were in the order of ATP > GTP > CTP > ITP > UTP for both the TAP1 and TAP2 C‐terminal domains, suggesting ATP to be the natural substrate of the transporter. Our data provide the first evidence that the individual C‐terminal domain of either TAP1 or TAP2 can be expressed as a functional ATP‐binding protein.