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Interaction of F‐actin with phosphate analogues studied by differential scanning calorimetry
Author(s) -
Nikolaeva Olga P.,
Dedova Irina V.,
Khvorova Irina S.,
Levitsky Dmitrii I.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00801-9
Subject(s) - differential scanning calorimetry , actin , chemistry , phosphate , calorimetry , crystallography , fluoride , thermal stability , biophysics , inorganic chemistry , biochemistry , organic chemistry , biology , physics , thermodynamics
The thermal unfolding of F‐actin and the changes induced in it by the binding of phosphate analogues were studied by differential scanning calorimetry. It is shown that the conformation of actin is drastically altered by interaction with beryllium fluoride or aluminium fluoride, while the effects of vanadate and phosphate are negligible. The effect of beryllium fluoride on the F‐actin structure, as reflected in a significant increase of the actin thermal stability, is much more pronounced in the presence of Mg 2+ than in the case of F‐actin polymerized by KCl or LiCl in the absence of Mg 2+ . It is concluded that differential scanning calorimetry is a very convenient method for probing the conformational changes in F‐actin caused by the interaction with phosphate analogues.