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Expression and regulation of human and rat phosphodiesterase type IV isogenes
Author(s) -
Engels Peter,
Fichtel Katharina,
Lübbert Hermann
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00788-8
Subject(s) - phosphodiesterase , rolipram , cell type , biology , human blood , gene , gene expression , cell , endocrinology , microbiology and biotechnology , medicine , enzyme , biochemistry , physiology
Type IV phosphodiesterases (PDE IV) specifically hydrolyze cAMP and are inhibited by rolipram. RT‐PCR was applied to analyze the expression patterns of mRNAs for four cloned human and rat phosphodiesterase type IV isogenes (PDE IV‐A, ‐B, ‐C and ‐D). Although these patterns were mostly coincident for the human and rat PDE IV genes, some differences were found between the two species. PDE IV‐A expression was detectable in human blood but not in rat blood, suggesting a species‐specific difference in the expression of this PDE IV isogene. PDE IV‐C was neither detected in human or rat blood nor in different cell populations of the human immune system. It is further demonstrated that the PDE IV isogene expression is differentially regulated by cAMP in different cell types.