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Heat‐shock response in a yeast tps1 mutant deficient in trehalose synthesis
Author(s) -
Argüelles Juan Carlos
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00786-1
Subject(s) - trehalose , mutant , yeast , saccharomyces cerevisiae , heat stress , biochemistry , wild type , chemistry , atp synthase , biology , enzyme , gene , zoology
Exponential cells of the Saccharomyces cerevisiae tps1 mutant underwent a rapid loss of viability upon a non‐lethal heat exposure (from 28 to 42°C). However, a further more severe heat stress (52.5°C 5 min) induced an increase in the fraction of viable cells. This mutant can not synthesize trehalose either at 28° C or at 42°C due to the lack of a functional trehalose‐6P synthase complex. In control experiments carried out with the wild‐type W303‐1 B, heat‐stressed exponential phase cultures grown on YPgal at 28°C acquired thermotolerance to a higher extent than identical cultures grown on YPD, although in both cultures the level of stored trehalose was negligible. These data suggest that the bulk of trehalose accumulated in yeast upon mild heat treaments is not sufficient to account for the acquisition of thermotolerance.