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Association of N ‐ethylmaleimide‐sensitive factor with synaptic vesicles
Author(s) -
Hong Rong-Mao,
Mori Hiroyuki,
Fukui Toshio,
Moriyama Yoshinori,
Futai Masamitsu,
Yamamoto Akitsugu,
Tashiro Yutaka,
Tagaya Mitsuo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00778-0
Subject(s) - synaptophysin , synaptic vesicle , vesicle , cerebellum , immunoprecipitation , microbiology and biotechnology , chemistry , vesicle fusion , vesicular transport protein , immunostaining , biophysics , biology , biochemistry , neuroscience , membrane , immunohistochemistry , immunology , gene
N ‐Ethylmaleimide‐sensitive factor (NSF) mediates docking and/or fusion of transport vesicles in the multi‐pathways of vesicular transport. NSF was highly expressed in brain and adrenal gland. Immunostaining of cerebellum with an anti‐NSF monoclonal antibody showed that NSF is predominantly localized in the molecular layers and the glomeruli of the granule cell layers. This distribution coincided well with that of synaptophysin, a marker protein of synaptic vesicles. Purification and immunoprecipitation revealed that NSF is associated with brain synaptic vesicles. The present results suggest that NSF is associated with synaptic vesicles without Ca 2+ influx.