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Differential inhibition of eukaryotic DNA polymerases by halenaquinol sulfate, a p ‐hydroquinone sulfate obtained from a marine sponge
Author(s) -
Shioda Masaki,
Kano Kazukaka,
Kobayashi Motomasa,
Kitagawa Isao,
Shoji Mami,
Yoshida Shonen,
Ikegami Susumu
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00777-2
Subject(s) - hydroquinone , sponge , sulfate , chemistry , dna , biochemistry , microbiology and biotechnology , biology , botany , organic chemistry
Halenaquinol sulfate, a p ‐hydroquinone sulfate obtained from a marine sponge, inhibited the activity of eukaryotic DNA polymerases in varying degrees; the K i values for DNA polymerases α, β, δ and ε were 1.3, 80, 17.5 and 2.0 νM, respectively, whereas it was less effective against E. coli DNA polymerase I. The inhibition occurred competitively with each of dATP and dTTP, but non‐competitively with dCTP, dGTP and the template DNA. Thus, halenaquinol sulfate is demonstrated to be a potential inhibitor of DNA polymerases α and ε, and be a useful tool for analysing the DNTP binding sites of DNA polymerases.