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The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase
Author(s) -
Cavallaro Antonino,
Ludwig Martha,
Burnell James
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00767-5
Subject(s) - transit peptide , carbonic anhydrase , complementary dna , biochemistry , chloroplast , biology , nucleic acid sequence , peptide sequence , cytosol , carbonic anhydrase i , enzyme , microbiology and biotechnology , botany , dna , gene , plastid
We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis , a C 4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C 3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C 3 plants) is not removed following translation of mRNA.