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Brefeldin A inhibits protein synthesis through the phosphorylation of the α‐subunit of eukaryotic initiation factor‐2
Author(s) -
Mellor Harry,
Kimball Scot R.,
Jefferson Leonard S.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00756-x
Subject(s) - brefeldin a , phosphorylation , protein subunit , initiation factor , eukaryotic initiation factor , microbiology and biotechnology , eif4a1 , chemistry , eif2 , biology , biochemistry , golgi apparatus , translation (biology) , endoplasmic reticulum , messenger rna , gene
Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371–374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide‐chain initiation through a mechanism involving the phosphorylation of the α‐subunit of eukaryotic initiation factor‐2 (eIF‐2α).