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Iron deprivation‐induced apoptosis in HL‐60 cells
Author(s) -
Fukuchi Kunihiko,
Tomoyasu Shigeru,
Tsuruoka Nobuyoshi,
Gomi Kunihide
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)00755-1
Subject(s) - apoptosis , dna fragmentation , deferoxamine , flow cytometry , fragmentation (computing) , microbiology and biotechnology , prophase , chemistry , chromatin , 8 hydroxy 2' deoxyguanosine , dna , dna damage , programmed cell death , biology , biochemistry , ecology , meiosis , gene , dna oxidation
Iron deprivation of HL‐60 cells with deferoxamine B mesylate (DFO) induced apoptosis. DNA fragmentation became apparent with 10 −6 M DFO after 48 h treatment. The apoptosis peak according to the DNA histogram on flow cytometory and typical nuclear collapse and were observed microscopically after 48 h treatment with 10 −4 M DFO. Cells treated with 10 −4 M DFO for as little as 24 h were shown to be committed to apoptosis, as chromatin condensation progressed gradually thereafter.